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EPC1/TIP60-Mediated Histone Acetylation Facilitates Spermiogenesis in Mice.

ABSTRACT: Global histone hyperacetylation is suggested to play a critical role for replacement of histones by transition proteins and protamines to compact the genome during spermiogenesis. However, the underlying mechanisms for hyperacetylation-mediated histone replacement remains poorly understood. Here, we report that EPC1 and TIP60, two critical components of the mammalian nucleosome acetyltransferase of H4 (NuA4) complexes, are coexpressed in male germ cells. Strikingly, genetic ablation of either Epc1 or Tip60 disrupts hyperacetylation and impairs histone replacement, in turn causing aberrant spermatid development. Taking these observations together, we reveal an essential role of the NuA4 complexes for histone hyperacetylation and subsequent compaction of the spermatid genome.

SUBMITTER: Dong Y

PROVIDER: S-EPMC5599718 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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EPC1/TIP60-Mediated Histone Acetylation Facilitates Spermiogenesis in Mice.

Dong Yixin Y Isono Kyo-Ichi KI Ohbo Kazuyuki K Endo Takaho A TA Ohara Osamu O Maekawa Mamiko M Toyama Yoshiro Y Ito Chizuru C Toshimori Kiyotaka K Helin Kristian K Ogonuki Narumi N Inoue Kimiko K Ogura Atsuo A Yamagata Kazutsune K Kitabayashi Issay I Koseki Haruhiko H

Molecular and cellular biology 20170912 19

Global histone hyperacetylation is suggested to play a critical role for replacement of histones by transition proteins and protamines to compact the genome during spermiogenesis. However, the underlying mechanisms for hyperacetylation-mediated histone replacement remains poorly understood. Here, we report that EPC1 and TIP60, two critical components of the mammalian nucleosome acetyltransferase of H4 (NuA4) complexes, are coexpressed in male germ cells. Strikingly, genetic ablation of either <i ...[more]

PMID: 28694333

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