Ontology highlight
ABSTRACT:
SUBMITTER: Han L
PROVIDER: S-EPMC5605247 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Han Lin L Wu Hua-Jun HJ Zhu Haiying H Kim Kun-Yong KY Marjani Sadie L SL Riester Markus M Euskirchen Ghia G Zi Xiaoyuan X Yang Jennifer J Han Jasper J Snyder Michael M Park In-Hyun IH Irizarry Rafael R Weissman Sherman M SM Michor Franziska F Fan Rong R Pan Xinghua X
Nucleic acids research 20170601 10
Conventional DNA bisulfite sequencing has been extended to single cell level, but the coverage consistency is insufficient for parallel comparison. Here we report a novel method for genome-wide CpG island (CGI) methylation sequencing for single cells (scCGI-seq), combining methylation-sensitive restriction enzyme digestion and multiple displacement amplification for selective detection of methylated CGIs. We applied this method to analyzing single cells from two types of hematopoietic cells, K56 ...[more]