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CpG island methylation

ABSTRACT: We compare the methylation status of CpG island clones by MeDIP in SW48 colon cancer cells relative to normal colon mucosa and WI38 primary fibroblasts.

ORGANISM(S): Homo sapiens

SUBMITTER: Wan Lam

PROVIDER: E-GEOD-2653 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chromosome-wide and promoter-specific analyses identify sites of differential DNA methylation in normal and transformed human cells.

Weber Michael M Davies Jonathan J JJ Wittig David D Oakeley Edward J EJ Haase Michael M Lam Wan L WL Schübeler Dirk D

Nature genetics 20050710 8

Cytosine methylation is required for mammalian development and is often perturbed in human cancer. To determine how this epigenetic modification is distributed in the genomes of primary and transformed cells, we used an immunocapturing approach followed by DNA microarray analysis to generate methylation profiles of all human chromosomes at 80-kb resolution and for a large set of CpG islands. In primary cells we identified broad genomic regions of differential methylation with higher levels in ge ...[more]

PMID: 16007088

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