Ontology highlight
ABSTRACT:
SUBMITTER: Ritzmann N
PROVIDER: S-EPMC5607040 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Biophysical journal 20170708 6
One major objective of synthetic biology is the bottom-up assembly of minimalistic nanocells consisting of lipid or polymer vesicles as architectural scaffolds and of membrane and soluble proteins as functional elements. However, there is no reliable method to orient membrane proteins reconstituted into vesicles. Here, we introduce a simple approach to orient the insertion of the light-driven proton pump proteorhodopsin (PR) into liposomes. To this end, we engineered red or green fluorescent pro ...[more]