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GuideScan software for improved single and paired CRISPR guide RNA design.

ABSTRACT: We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.

SUBMITTER: Perez AR

PROVIDER: S-EPMC5607865 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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GuideScan software for improved single and paired CRISPR guide RNA design.

Perez Alexendar R AR Pritykin Yuri Y Vidigal Joana A JA Chhangawala Sagar S Zamparo Lee L Leslie Christina S CS Ventura Andrea A

Nature biotechnology 20170306 4

We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools. ...[more]

PMID: 28263296

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