Ontology highlight
ABSTRACT:
SUBMITTER: Wiley LA
PROVIDER: S-EPMC5609845 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Wiley Luke A LA Anfinson Kristin R KR Cranston Cathryn M CM Kaalberg Emily E EE Collins Malia M MM Mullins Robert F RF Stone Edwin M EM Tucker Budd A BA
Current protocols in stem cell biology 20170814
This unit describes protocols for the generation of clinical-grade patient-specific induced pluripotent stem cell (iPSC)-derived retinal cells from patients with inherited retinal degenerative blindness. Specifically, we describe how, using xeno-free reagents in an ISO class 5 environment, one can isolate and culture dermal fibroblasts, generate iPSCs, and derive autologous retinal cells via 3-D differentiation. The universal methods described herein for the isolation of dermal fibroblasts and g ...[more]