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Generation of Xeno-Free, cGMP-Compliant Patient-Specific iPSCs from Skin Biopsy.


ABSTRACT: This unit describes protocols for the generation of clinical-grade patient-specific induced pluripotent stem cell (iPSC)-derived retinal cells from patients with inherited retinal degenerative blindness. Specifically, we describe how, using xeno-free reagents in an ISO class 5 environment, one can isolate and culture dermal fibroblasts, generate iPSCs, and derive autologous retinal cells via 3-D differentiation. The universal methods described herein for the isolation of dermal fibroblasts and generation of iPSCs can be employed regardless of disease, tissue, or cell type of interest. © 2017 by John Wiley & Sons, Inc.

SUBMITTER: Wiley LA 

PROVIDER: S-EPMC5609845 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Generation of Xeno-Free, cGMP-Compliant Patient-Specific iPSCs from Skin Biopsy.

Wiley Luke A LA   Anfinson Kristin R KR   Cranston Cathryn M CM   Kaalberg Emily E EE   Collins Malia M MM   Mullins Robert F RF   Stone Edwin M EM   Tucker Budd A BA  

Current protocols in stem cell biology 20170814


This unit describes protocols for the generation of clinical-grade patient-specific induced pluripotent stem cell (iPSC)-derived retinal cells from patients with inherited retinal degenerative blindness. Specifically, we describe how, using xeno-free reagents in an ISO class 5 environment, one can isolate and culture dermal fibroblasts, generate iPSCs, and derive autologous retinal cells via 3-D differentiation. The universal methods described herein for the isolation of dermal fibroblasts and g  ...[more]

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