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Resveratrol inhibits plasma membrane Ca2+-ATPase inducing an increase in cytoplasmic calcium.


ABSTRACT: Plasma membrane Ca2+-ATPase (PMCA) plays a vital role in maintaining cytosolic calcium concentration ([Ca2+] i ). Given that many diseases have modified PMCA expression and activity, PMCA is an important potential target for therapeutic treatment. This study demonstrates that the non-toxic, naturally-occurring polyphenol resveratrol (RES) induces increases in [Ca2+] i via PMCA inhibition in primary dermal fibroblasts and MDA-MB-231 breast cancer cells. Our results also illustrate that RES and the fluorescent intracellular calcium indicator Fura-2, are compatible for simultaneous use, in contrast to previous studies, which indicated that RES modulates the Fura-2 fluorescence independent of calcium concentration. Because RES has been identified as a PMCA inhibitor, further studies may be conducted to develop more specific PMCA inhibitors from RES derivatives for potential therapeutic use.

SUBMITTER: Peterson JA 

PROVIDER: S-EPMC5613515 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Resveratrol inhibits plasma membrane Ca<sup>2+</sup>-ATPase inducing an increase in cytoplasmic calcium.

Peterson Joshua Allen JA   Oblad Richard Vernon RV   Mecham Jeffrey Chad JC   Kenealey Jason Donald JD  

Biochemistry and biophysics reports 20160630


Plasma membrane Ca<sup>2+</sup>-ATPase (PMCA) plays a vital role in maintaining cytosolic calcium concentration ([Ca<sup>2+</sup>] <sub><i>i</i></sub> ). Given that many diseases have modified PMCA expression and activity, PMCA is an important potential target for therapeutic treatment. This study demonstrates that the non-toxic, naturally-occurring polyphenol resveratrol (RES) induces increases in [Ca<sup>2+</sup>] <sub><i>i</i></sub> via PMCA inhibition in primary dermal fibroblasts and MDA-MB  ...[more]

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