Ontology highlight
ABSTRACT:
SUBMITTER: Szilvay AM
PROVIDER: S-EPMC5613695 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Szilvay Anne-Marie AM Sarria Shirley Vanessa SV Mannelqvist Monica M Aasland Rein R Furnes Clemens C
Biochemistry and biophysics reports 20160716
An approach to assay proteolytic activity <i>in vivo</i> by altering the subcellular localization of a labelled substrate was demonstrated. The assay included a protein shuttling between different cellular compartments and a site-specific recombinant protease. The shuttle protein used was the human immunodeficiency virus type 1 (HIV-1) Rev protein tandemly fused to the enhanced green fluorescent protein (EGFP) and the red fluorescent protein (RFP), while the protease was the site-specific protea ...[more]