Ontology highlight
ABSTRACT:
SUBMITTER: Trettin KD
PROVIDER: S-EPMC5617286 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20170905 38
Loquacious-PD (Loqs-PD) is required for biogenesis of many endogenous siRNAs in <i>Drosophila</i> In vitro, Loqs-PD enhances the rate of dsRNA cleavage by Dicer-2 and also enables processing of substrates normally refractory to cleavage. Using purified components, and Loqs-PD truncations, we provide a mechanistic basis for Loqs-PD functions. Our studies indicate that the 22 amino acids at the C terminus of Loqs-PD, including an FDF-like motif, directly interact with the Hel2 subdomain of Dicer-2 ...[more]