Regulating miRNA-21 Biogenesis By Bifunctional Small Molecules.

ABSTRACT: We report a new strategy to regulate microRNAs (miRNAs) biogenesis by using bifunctional small molecules that consist of a pre-miRNA binding unit connected by a linker to a Dicer inhibiting unit. In this effort, fluorescence polarization-based screening was used to identify neomycin as a pre-miR-21 binding ligand. Although neomycin cannot inhibit miR-21 maturation, linking it to the RNase inhibitor 1 forms the bifunctional conjugate 7A, which inhibits the production of miR-21. We expect that this strategy will be applicable to design other molecules for miRNA regulation.

SUBMITTER: Yan H 

PROVIDER: S-EPMC5620130 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature