Dataset Information

Convolutional neural networks for automated annotation of cellular cryo-electron tomograms.

ABSTRACT: Cellular electron cryotomography offers researchers the ability to observe macromolecules frozen in action in situ, but a primary challenge with this technique is identifying molecular components within the crowded cellular environment. We introduce a method that uses neural networks to dramatically reduce the time and human effort required for subcellular annotation and feature extraction. Subsequent subtomogram classification and averaging yield in situ structures of molecular components of interest. The method is available in the EMAN2.2 software package.

SUBMITTER: Chen M

PROVIDER: S-EPMC5623144 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Publications

Convolutional neural networks for automated annotation of cellular cryo-electron tomograms.

Chen Muyuan M Dai Wei W Sun Stella Y SY Jonasch Darius D He Cynthia Y CY Schmid Michael F MF Chiu Wah W Ludtke Steven J SJ

Nature methods 20170828 10

Cellular electron cryotomography offers researchers the ability to observe macromolecules frozen in action in situ, but a primary challenge with this technique is identifying molecular components within the crowded cellular environment. We introduce a method that uses neural networks to dramatically reduce the time and human effort required for subcellular annotation and feature extraction. Subsequent subtomogram classification and averaging yield in situ structures of molecular components of in ...[more]

PMID: 28846087

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Project description:BackgroundDespite recent advances in cellular cryo-electron tomography (CET), developing automated tools for macromolecule identification in submolecular resolution remains challenging due to the lack of annotated data and high structural complexities. To date, the extent of the deep learning methods constructed for this problem is limited to conventional Convolutional Neural Networks (CNNs). Identifying macromolecules of different types and sizes is a tedious and time-consuming task. In this paper, we employ a capsule-based architecture to automate the task of macromolecule identification, that we refer to as 3D-UCaps. In particular, the architecture is composed of three components: feature extractor, capsule encoder, and CNN decoder. The feature extractor converts voxel intensities of input sub-tomograms to activities of local features. The encoder is a 3D Capsule Network (CapsNet) that takes local features to generate a low-dimensional representation of the input. Then, a 3D CNN decoder reconstructs the sub-tomograms from the given representation by upsampling.ResultsWe performed binary and multi-class localization and identification tasks on synthetic and experimental data. We observed that the 3D-UNet and the 3D-UCaps had an [Formula: see text]score mostly above 60% and 70%, respectively, on the test data. In both network architectures, we observed degradation of at least 40% in the [Formula: see text]-score when identifying very small particles (PDB entry 3GL1) compared to a large particle (PDB entry 4D8Q). In the multi-class identification task of experimental data, 3D-UCaps had an [Formula: see text]-score of 91% on the test data in contrast to 64% of the 3D-UNet. The better [Formula: see text]-score of 3D-UCaps compared to 3D-UNet is obtained by a higher precision score. We speculate this to be due to the capsule network employed in the encoder. To study the effect of the CapsNet-based encoder architecture further, we performed an ablation study and perceived that the [Formula: see text]-score is boosted as network depth is increased which is in contrast to the previously reported results for the 3D-UNet. To present a reproducible work, source code, trained models, data as well as visualization results are made publicly available.ConclusionQuantitative and qualitative results show that 3D-UCaps successfully perform various downstream tasks including identification and localization of macromolecules and can at least compete with CNN architectures for this task. Given that the capsule layers extract both the existence probability and the orientation of the molecules, this architecture has the potential to lead to representations of the data that are better interpretable than those of 3D-UNet.

Dataset Information

Convolutional neural networks for automated annotation of cellular cryo-electron tomograms.

Publications

Convolutional neural networks for automated annotation of cellular cryo-electron tomograms.

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