Ontology highlight
ABSTRACT:
SUBMITTER: Hecksel CW
PROVIDER: S-EPMC5111626 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada 20160526 3
Although acknowledged to be variable and subjective, manual annotation of cryo-electron tomography data is commonly used to answer structural questions and to create a "ground truth" for evaluation of automated segmentation algorithms. Validation of such annotation is lacking, but is critical for understanding the reproducibility of manual annotations. Here, we used voxel-based similarity scores for a variety of specimens, ranging in complexity and segmented by several annotators, to quantify th ...[more]