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Use of RNALater® Preservation for Virome Sequencing in Outbreak Settings.


ABSTRACT: Outbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. During outbreak situations, straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for metagenomic virus detection in clinical specimens (TUViD-VM) was developed for snap-frozen tissues which can be challenging to obtain. Here, we describe the use of RNALater® -treated tissue at ambient temperatures for virome sequencing. This study demonstrates that samples stored in RNALater® buffer yield similar results to those stored snap-frozen.

SUBMITTER: Kohl C 

PROVIDER: S-EPMC5623189 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Use of <i>RNALater</i><sup>®</sup> Preservation for Virome Sequencing in Outbreak Settings.

Kohl Claudia C   Wegener Merle M   Nitsche Andreas A   Kurth Andreas A  

Frontiers in microbiology 20170926


Outbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. During outbreak situations, straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for metagenomic virus detection in clinical specimens (TUViD-VM) was developed for snap-frozen tissues which can be challenging to obtain. Here, we describe the use of <i>RNALater<sup>®</sup></i> -treated ti  ...[more]

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