Dataset Information

Conformational analysis of a synthetic fish kisspeptin 1 peptide in membrane mimicking environments.

ABSTRACT: Kisspeptin 1 is a neuropeptide hormone of the RFamide family, which act as an upstream regulator of brain-pituitary-gonad (BPG) axis in most vertebrates including teleosts. In the present study, a 16 amino acid long putative mature bioactive peptide (kiss 1) from preprokisspeptin 1 of golden mahseer, Tor putitora (Hamilton, 1822), was synthesized and characterized using an integrated (experimental and in silico) approach. The far-UV circular dichroism (CD) spectrum of this peptide was evaluated both in aqueous and membrane mimicking solvents (TFE, HFIP and Dioxane). The results indicate that kiss 1 peptide adopted helical, turn and ? conformations in membrane like environments. The near-UV CD spectroscopy was also carried out to examine the tertiary packing around aromatic residues of kiss 1 peptide and the peptide-membrane complex. The kiss 1 peptide exhibited little signal in water, but a prominent negative band was observed at around 275 nm when membrane mimetic solution was added. The observed ordered conformations of kiss 1 peptide in the different solvents indicated its potential biological activity which could enhance the secretion of gonadotropin-releasing hormone (GnRH) at BPG axis. The conformational information generated from the present study reinforces the application prospects of bioactive synthetic peptide analogs of kisspeptin 1 in improving the reproductive performances of important cultivable fish species.

SUBMITTER: Thakuria D

PROVIDER: S-EPMC5627949 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Conformational analysis of a synthetic fish kisspeptin 1 peptide in membrane mimicking environments.

Thakuria Dimpal D Shahi Neetu N Singh Atul Kumar AK Khangembam Victoria Chanu VC Singh Arvind Kumar AK Kumar Satish S

PloS one 20171004 10

Kisspeptin 1 is a neuropeptide hormone of the RFamide family, which act as an upstream regulator of brain-pituitary-gonad (BPG) axis in most vertebrates including teleosts. In the present study, a 16 amino acid long putative mature bioactive peptide (kiss 1) from preprokisspeptin 1 of golden mahseer, Tor putitora (Hamilton, 1822), was synthesized and characterized using an integrated (experimental and in silico) approach. The far-UV circular dichroism (CD) spectrum of this peptide was evaluated ...[more]

PMID: 28977030

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Dataset Information

Conformational analysis of a synthetic fish kisspeptin 1 peptide in membrane mimicking environments.

Publications

Conformational analysis of a synthetic fish kisspeptin 1 peptide in membrane mimicking environments.

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