Ontology highlight
ABSTRACT:
SUBMITTER: Varshney GK
PROVIDER: S-EPMC5630457 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Varshney Gaurav K GK Carrington Blake B Pei Wuhong W Bishop Kevin K Chen Zelin Z Fan Chunxin C Xu Lisha L Jones Marypat M LaFave Matthew C MC Ledin Johan J Sood Raman R Burgess Shawn M SM
Nature protocols 20161027 12
The zebrafish is a popular model organism for studying development and disease, and genetically modified zebrafish provide an essential tool for functional genomic studies. Numerous publications have demonstrated the efficacy of gene targeting in zebrafish using CRISPR/Cas9, and they have included descriptions of a variety of tools and methods for guide RNA synthesis and mutant identification. However, most of the published techniques are not readily scalable to increase throughput. We recently ...[more]