Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ.

ABSTRACT: Condensins I and II are multisubunit complexes that play a central role in mitotic chromosome assembly. Although both complexes become concentrated along the axial region of each chromatid by metaphase, it remains unclear exactly how such axes might assemble and contribute to chromosome shaping. To address these questions from a physico-chemical point of view, we have established a set of two-step protocols for inducing reversible assembly of chromosome structure in situ, namely within a whole cell. In this assay, mitotic chromosomes are first expanded in a hypotonic buffer containing a Mg²⁺-chelating agent and then converted into different shapes in a NaCl concentration-dependent manner. Both chromatin and condensin-positive chromosome axes are converted into near-original shap

SUBMITTER: Ono T 

PROVIDER: S-EPMC5638589 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature