Dataset Information

Variants of GSK3? and SFRP4 genes in Wnt signaling were not associated with osteonecrosis of the femoral head.

ABSTRACT: Genome-wide association studies have identified that the gene variants in Wnt signaling associate with bone mineral density and fracture risk but the effects of the variants on the development of osteonecrosis of the femoral head (ONFH) have been unclear. Here, we analyzed the polymorphisms of 4 variants in GSK3? and SFRP4 genes of Wnt signaling and their association with the development of ONFH through Mass ARRAY® platform in 200 ONFH patients and 177controls in Chinese population. Our results showed that the genotypes and allele frequencies of all variants detected in SFRP4 and GSK3? genes were not significantly different between patients and controls (p > 0.05); the correlation analysis between the 4 variants genotypes and gender, age at onset, etiological classification, unilateral or bilateral hip lesions, and clinical stages of ONFH, respectively, did not confirm significant association (p > 0.05) although age at onset in the minor homozygous(CC) carriers of SFRP4 rs1052981 (T/C) was a statistically younger tendency than that of the major homozygous (TT) or heterozygous (TC) of the SNP (p = 0.051); moreover, all haplotypes analyzed and their association with the clinical phenotypes of ONFH were also shown no statistical significance (p > 0.05).These results suggest that the 4 variants analyzed by this study in GSK3? and SFRP4 genes of Wnt signaling pathway are unlikely to be associated with susceptibility to ONFH.

SUBMITTER: Song Y

PROVIDER: S-EPMC5641138 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Variants of GSK3β and SFRP4 genes in Wnt signaling were not associated with osteonecrosis of the femoral head.

Song Yang Y Du Zhenwu Z Yang Qiwei Q Ren Ming M Wang Qingyu Q Chen Gaoyang G Zhao Haiyue H Li Zhaoyan Z Zhang Guizhen G

Oncotarget 20170822 42

Genome-wide association studies have identified that the gene variants in Wnt signaling associate with bone mineral density and fracture risk but the effects of the variants on the development of osteonecrosis of the femoral head (ONFH) have been unclear. Here, we analyzed the polymorphisms of 4 variants in GSK3β and SFRP4 genes of Wnt signaling and their association with the development of ONFH through Mass ARRAY® platform in 200 ONFH patients and 177controls in Chinese ...[more]

PMID: 29069795

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Project description:BackgroundGlucocorticoid usage and alcohol abuse are the most widely accepted risk factors for nontraumatic osteonecrosis of femoral head (ONFH). Despite distinct etiologies between glucocorticoid-associated ONFH (GONFH) and alcohol-associated ONFH (AONFH), little is known about the differences of the microarchitectural and histomorphologic characteristics between these subtypes of ONFH.PurposesTo investigate bone microarchitecture, bone remodeling activity and histomorphology characteristics of different regions in femoral heads between GONFH and AONFH.MethodsFrom September 2015 to October 2020, 85 patients diagnosed with GONFH and AONFH were recruited. Femoral heads were obtained after total hip replacement. Femoral head specimens were obtained from 42 patients (50 hips) with GONFH and 43 patients (50 hips) with AONFH. Micro-CT was utilized to assess the microstructure of 9 regions of interest (ROIs) in the femoral head. Along the supero-inferior orientation, the femoral head was divided into necrotic region, reactive interface, and normal region; along the medio-lateral orientation, the femoral head was divided into medial region, central region and lateral region. Decalcified and undecalcified bone histology was subsequently performed to evaluate histopathological alterations and bone remodeling levels.ResultsIn the necrotic region, most of the microarchitectural parameters did not differ significantly between GONFH and AONFH, whereas both the reactive interface and normal region revealed a less sclerotic microarchitecture but a higher bone remodeling level in GONFH than AONFH. Despite similar necrotic pathological manifestations, subchondral trabecular microfracture in the necrotic region was more severe and vasculature of the reactive interface was more abundant in GONFH.ConclusionsGONFH and AONFH shared similar microarchitecture and histopathological features in the necrotic region, while GONFH exhibited a less sclerotic microarchitecture and a more active bone metabolic status in both the reactive interface and normal region. These differences between GONFH and AONFH in bone microarchitectural and histopathological characteristics might contribute to the development of disease-modifying prevention strategies and treatments for ONFH, taking into etiologies.

Project description:BackgroundSteroid-induced osteonecrosis of the femoral head (SONFH) is a disorder that causes severe disability in patients and has a high incidence worldwide. Although glucocorticoid (GC)-induced apoptosis of osteoblasts is an important cytological basis of SONFH, the detailed mechanism underlying SONFH pathogenesis remains elusive. PI3K/AKT signaling pathway was reported to involve in cell survival and apoptosis.ObjectiveWe explored the role of PI3K/AKT/FOXO1 signaling pathway and its downstream targets during glucocorticoid -induced osteonecrosis of the femoral head.MethodsWe obtained gene expression profile of osteoblasts subjected to dexamethasone (Dex) treatment from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were screened out and functional enrichment analysis were conducted by bioinformatics analysis. In vitro, we analyzed Dex-induced apoptosis in MC3T3-E1 cells and explored the role of PI3K/AKT/FOXO1 signaling pathway in this phenomenon by employing siRNA-FOXO1 and IGF-1(PI3K/AKT agonist). Finally, we verified our results in a rat model of SONFH.ResultsIn Dex-treated osteoblasts, DEGs were mainly enriched in the FOXO signaling pathway. Dex inhibited MC3T3-E1 cell viability in a dose-dependent effect and induced apoptosis by increasing the expression levels of FOXO1, Bax, cleaved-Caspase-3, and cleaved-Caspase-9, while reducing the expression of Bcl-2. Notably, these results were reversed by siRNA-FOXO1 treatment. Dex inhibited PI3K/AKT signaling pathway, upregulated FOXO1 expression and increased FOXO1 nuclear translocation, which were reversed by IGF-1. Compared to normal rats, the femoral head of SONFH showed increased expression of FOXO1, increased number of apoptotic cells, and empty osteocytic lacunas, as well as decreased bone tissue content and femoral head integrity. Significantly, the effects of GC-induced SONFH were alleviated following IGF-1 treatment.ConclusionDex induces osteoblast apoptosis via the PI3K/AKT/FOXO1 signaling pathway. Our research offers new insights into the underlying molecular mechanisms of glucocorticoid-induced osteonecrosis in SONFH and proposes FOXO1 as a therapeutic target for this disease.

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Variants of GSK3? and SFRP4 genes in Wnt signaling were not associated with osteonecrosis of the femoral head.

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Variants of <i>GSK3β</i> and <i>SFRP4</i> genes in Wnt signaling were not associated with osteonecrosis of the femoral head.

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