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A calibration routine for efficient ETD in large-scale proteomics.

ABSTRACT: Electron transfer dissociation (ETD) has been broadly adopted and is now available on a variety of commercial mass spectrometers. Unlike collisional activation techniques, optimal performance of ETD requires considerable user knowledge and input. ETD reaction duration is one key parameter that can greatly influence spectral quality and overall experiment outcome. We describe a calibration routine that determines the correct number of reagent anions necessary to reach a defined ETD reaction rate. Implementation of this automated calibration routine on two hybrid Orbitrap platforms illustrate considerable advantages, namely, increased product ion yield with concomitant reduction in scan rates netting up to 75% more unique peptide identifications in a shotgun experiment. Graphical Abstract ?.

SUBMITTER: Rose CM

PROVIDER: S-EPMC5642106 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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A calibration routine for efficient ETD in large-scale proteomics.

Rose Christopher M CM Rush Matthew J P MJ Riley Nicholas M NM Merrill Anna E AE Kwiecien Nicholas W NW Holden Dustin D DD Mullen Christopher C Westphall Michael S MS Coon Joshua J JJ

Journal of the American Society for Mass Spectrometry 20150626 11

Electron transfer dissociation (ETD) has been broadly adopted and is now available on a variety of commercial mass spectrometers. Unlike collisional activation techniques, optimal performance of ETD requires considerable user knowledge and input. ETD reaction duration is one key parameter that can greatly influence spectral quality and overall experiment outcome. We describe a calibration routine that determines the correct number of reagent anions necessary to reach a defined ETD reaction rate. ...[more]

PMID: 26111518

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