Unknown

Dataset Information

0

Tumor-infiltrating B cells producing antitumor active immunoglobulins in resected HCC prolong patient survival.


ABSTRACT: The immunological microenvironment of HCC influences patient outcome, however, the role of B cells remains unclear. This study investigated effects of local B-cell infiltration in HCC cohorts on patient survival and immunological and molecular tumor microenvironment.Unsupervised gene expression analysis of full cancer transcriptomes (N=2158) revealed a highly co-regulated immunological cluster in HCC that mainly contained immunoglobulin fragments. More specifically, in an independent patient cohort (N=242) that compares HCC with non tumorous liver tissue high expression of these B-cell associated genes was associated with better patient outcome (P=0.0149). Conclusively, the immunohistochemical analysis of another independent cohort of resected HCCs (N=119) demonstrated that infiltration of HCCs by CD20+ cells (P=0.004) and CD79a+ cells (P=0.038) at the infiltrative margin were associated with prolonged patient survival. Further, the immunoglobulin fragments that were identified in the gene expression analysis were detected at high levels in patients with dense B-cell infiltration.Gene expression of 2 independent HCC tissue databases was compared using microarrays. Additionally, tissue of resected HCCs was stained for CD20, CD79a and immunoglobulins and analysed for the respective cell numbers separately for tumor, infiltrative margin and distant liver stroma. These findings were correlated with clinical data and patient outcome.Infiltration of HCCs by B cells is associated with prolonged patient survival. Further, a distinct B-cell like immunoglobulin profile of HCCs was identified that goes along with better patient outcome. We suggest that B cells contribute to local tumor control by secreting increased levels of immunoglobulins with antitumor activity.

SUBMITTER: Brunner SM 

PROVIDER: S-EPMC5642613 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Tumor-infiltrating B cells producing antitumor active immunoglobulins in resected HCC prolong patient survival.

Brunner Stefan M SM   Itzel Timo T   Rubner Christoph C   Kesselring Rebecca R   Griesshammer Eva E   Evert Matthias M   Teufel Andreas A   Schlitt Hans J HJ   Fichtner-Feigl Stefan S  

Oncotarget 20170809 41


<h4>Background & aims</h4>The immunological microenvironment of HCC influences patient outcome, however, the role of B cells remains unclear. This study investigated effects of local B-cell infiltration in HCC cohorts on patient survival and immunological and molecular tumor microenvironment.<h4>Results</h4>Unsupervised gene expression analysis of full cancer transcriptomes (N=2158) revealed a highly co-regulated immunological cluster in HCC that mainly contained immunoglobulin fragments. More s  ...[more]

Similar Datasets

2014-12-08 | E-GEOD-63850 | biostudies-arrayexpress
2014-12-08 | GSE63850 | GEO
| S-EPMC7645373 | biostudies-literature
| S-EPMC9818493 | biostudies-literature
| S-EPMC6504616 | biostudies-literature
| S-EPMC3959864 | biostudies-literature
| S-EPMC7081270 | biostudies-literature
| S-EPMC6038086 | biostudies-literature
| S-EPMC8656728 | biostudies-literature
| S-EPMC5850948 | biostudies-literature