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Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation.

ABSTRACT: Mammalian haploid embryonic stem cells (haESCs) provide new possibilities for large-scale genetic screens because they bear only one copy of each chromosome. However, haESCs are prone to spontaneous diploidization through unknown mechanisms. Here, we report that a small molecule combination could restrain mouse haESCs from diploidization by impeding exit from naïve pluripotency and by shortening the S-G2/M phases. Combined with 2i and PD166285, our chemical cocktail could maintain haESCs in the haploid state for at least five weeks without fluorescence-activated cell sorting (FACS) enrichment of haploid cells. Taken together, we established an effective chemical approach for long-term maintenance of haESCs, and highlighted that proper cell cycle progression was critical for the maintenance of haploid state.

SUBMITTER: Li H

PROVIDER: S-EPMC5643530 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Stabilization of mouse haploid embryonic stem cells with combined kinase and signal modulation.

Li Haisen H Guo Ao A Xie Zhenfei Z Tu Wanzhi W Yu Jiali J Wang Huihan H Zhao Jian J Zhong Cuiqing C Kang Jiuhong J Li Jinsong J Huang Shichao S Shen Li L

Scientific reports 20171016 1

Mammalian haploid embryonic stem cells (haESCs) provide new possibilities for large-scale genetic screens because they bear only one copy of each chromosome. However, haESCs are prone to spontaneous diploidization through unknown mechanisms. Here, we report that a small molecule combination could restrain mouse haESCs from diploidization by impeding exit from naïve pluripotency and by shortening the S-G2/M phases. Combined with 2i and PD166285, our chemical cocktail could maintain haESCs in the ...[more]

PMID: 29038567

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