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A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids.

ABSTRACT: Highly selective probes hybridize only to fully complementary DNA or RNA sequences and, therefore, often fail to recognize mutated viral genomes. Here we designed a probe that possesses two seemingly incompatible properties: it tolerates some point mutations in genome, while it remains selective towards others. An OR deoxyribozyme logic gate was designed to fluorescently report the sequences of enterovirus 71 (EV71) covering ?90% of all known EV71 strains. Importantly, sequences of closely related coxsackieviruses that differed by single nucleotides were reliably differentiated in 7 out of 8 cases.

SUBMITTER: Kamar O

PROVIDER: S-EPMC5645154 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids.

Kamar Ola O Sun Sin-Cih SC Lin Chieh-Hua CH Chung Wan-Yu WY Lee Min-Shi MS Liao Yu-Chieh YC Kolpashchikov Dmitry M DM Chuang Min-Chieh MC

Chemical communications (Cambridge, England) 20170901 76

Highly selective probes hybridize only to fully complementary DNA or RNA sequences and, therefore, often fail to recognize mutated viral genomes. Here we designed a probe that possesses two seemingly incompatible properties: it tolerates some point mutations in genome, while it remains selective towards others. An OR deoxyribozyme logic gate was designed to fluorescently report the sequences of enterovirus 71 (EV71) covering ∼90% of all known EV71 strains. Importantly, sequences of closely relat ...[more]

PMID: 28900642

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