Mapping Cell Membrane Fluctuations Reveals Their Active Regulation and Transient Heterogeneities.

ABSTRACT: Shape fluctuations of the plasma membrane occur in all cells, are incessant, and are proposed to affect membrane functioning. Although studies show how membrane fluctuations are affected by cellular activity in adherent cells, their spatial regulation and the corresponding change in membrane mechanics remain unclear. In this article, we study how ATP-driven activities and actomyosin cytoskeleton impact basal membrane fluctuations in adherent cells. Using interference imaging, we map height fluctuations within single cells and compare the temporal spectra with existing theoretical models to gain insights about the underlying membrane mechanics. We find that ATP-dependent activities enhance the nanoscale z fluctuations but stretch out the membrane laterally. Although actin polymerization or

SUBMITTER: Biswas A 

PROVIDER: S-EPMC5647594 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature