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Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity.


ABSTRACT: The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings and modeling of synaptic activity at cerebellar granule cell to Purkinje cell synapses of mice, we describe two sub-pools in the RRP that can be differentially recruited upon ultrafast changes in the stimulation frequency. We show that at low-frequency stimulations, a first sub-pool is gradually silenced, leading to full blockage of synaptic transmission. Conversely, a second pool of synaptic vesicles that cannot be released by a single stimulus is recruited within milliseconds by high-frequency stimulation and support an ultrafast recovery of neurotransmitter release after low-frequency depression. This frequency-dependent mobilization or silencing of sub-pools in the RRP in terminals of granule cells may play a role in the filtering of sensorimotor information in the cerebellum.

SUBMITTER: Doussau F 

PROVIDER: S-EPMC5648531 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity.

Doussau Frédéric F   Schmidt Hartmut H   Dorgans Kevin K   Valera Antoine M AM   Poulain Bernard B   Isope Philippe P  

eLife 20171009


The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-pools that are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings and modeling of synaptic activity at cerebellar granule cell to Purkinje cell synapses of mice, we describe two sub-pools in the RRP that can be differentially recruited upon ultrafast changes in the stimulation frequency. We s  ...[more]

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