Ontology highlight
ABSTRACT:
SUBMITTER: Demo G
PROVIDER: S-EPMC5655137 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Demo Gabriel G Rasouly Aviram A Vasilyev Nikita N Svetlov Vladimir V Loveland Anna B AB Diaz-Avalos Ruben R Grigorieff Nikolaus N Nudler Evgeny E Korostelev Andrei A AA
eLife 20171013
In bacteria, mRNA transcription and translation are coupled to coordinate optimal gene expression and maintain genome stability. Coupling is thought to involve direct interactions between RNA polymerase (RNAP) and the translational machinery. We present cryo-EM structures of <i>E. coli</i> RNAP core bound to the small ribosomal 30S subunit. The complex is stable under cell-like ionic conditions, consistent with functional interaction between RNAP and the 30S subunit. The RNA exit tunnel of RNAP ...[more]