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Abiotic stresses influence the transcript abundance of PIP and TIP aquaporins in Festuca species.

ABSTRACT: Festuca arundinacea and F. pratensis are the models in forage grasses to recognize the molecular basis of drought, salt and frost tolerance, respectively. Transcription profiles of plasma membrane intrinsic proteins (PIPs) and tonoplast intrinsic proteins (TIPs) aquaporin genes were obtained for leaves of Festuca species treated with different abiotic stimuli. F. arundinacea plants were exposed to drought and salt stress, whereas F. pratensis plants were cold-hardened. Changes in genes expression measured with use of real time qRT-PCR method were compared between two genotypes characterized with a significantly different level of each stress tolerance. Under drought the transcript level of PIP1;2 and TIP1;1 aquaporin decreased in both analyzed F. arundinacea genotypes, whereas for PIP2;1 only in a high drought tolerant plant. A salt treatment caused a reduction of PIP1;2 transcript level in a high salt tolerant genotype and an increase of TIP1;1 transcript abundance in both F. arundinacea genotypes, but it did not influence the expression of PIP2;1 aquaporin. During cold-hardening a decrease of PIP1;2, PIP2;1, and TIP1;1 aquaporin transcripts was observed, both in high and low frost tolerant genotypes. The obtained results revealed that the selected genotypes responded in a different way to abiotic stresses application. A reduced level of PIP1;2 transcript in F. arundinacea low drought tolerant genotype corresponded with a faster water loss and a lowering of photosynthesis efficiency and gas exchange during drought conditions. In F. pratensis, cold acclimation was associated with a lower level of aquaporin transcripts in both high and low frost tolerant genotypes. This is the first report on aquaporin transcriptional profiling under abiotic stress condition in forage grasses.

SUBMITTER: Pawlowicz I

PROVIDER: S-EPMC5655603 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Abiotic stresses influence the transcript abundance of PIP and TIP aquaporins in Festuca species.

Pawłowicz Izabela I Rapacz Marcin M Perlikowski Dawid D Gondek Krzysztof K Kosmala Arkadiusz A

Journal of applied genetics 20170804 4

Festuca arundinacea and F. pratensis are the models in forage grasses to recognize the molecular basis of drought, salt and frost tolerance, respectively. Transcription profiles of plasma membrane intrinsic proteins (PIPs) and tonoplast intrinsic proteins (TIPs) aquaporin genes were obtained for leaves of Festuca species treated with different abiotic stimuli. F. arundinacea plants were exposed to drought and salt stress, whereas F. pratensis plants were cold-hardened. Changes in genes expressio ...[more]

PMID: 28779288

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Project description:Aims:According to traditional ecophysiological theories stress tolerance of plants is predominately determined by universal physiochemical constraints. Plant acclimation to environmental stress therefore compromises plant performance under a different stress, hindering successful toleration of several abiotic stress factors simultaneously. Yet, recent studies have shown that these trade-offs are less exclusive than postulated so far, leaving more wiggle room for gaining polytolerance through adaptations We tested whether the polytolerance to shade and drought depends on cold and waterlogging tolerances - hypothesizing that polytolerance patterns in different species groups (angiosperms vs. gymnosperms; deciduous vs. evergreen; species originating from North America, Europe and East Asia) depend on the length of the vegetation period and species's dormancy through limiting the duration of favourable growing season. Location:Northern hemisphere. Methods:Our study analyzed four main abiotic stress factors - shade, drought, cold and waterlogging stress - for 806 Northern hemisphere woody species using cross-calibrated tolerance rankings. The importance of trade-offs among species ecological potentials was evaluated using the species-specific estimates of polytolerance to chosen factors. Results:We found that both cold and waterlogging tolerance are negatively related to species' capabilities of simultaneously tolerating low light and water conditions. While this pattern was different in angiosperms and gymnosperms, species region of origin and leaf type had no effect on this relationship. Main conclusions:Our results demonstrate that adaptation to different abiotic stress factors in woody plants is highly complex. Vegetation period length and dormancy are the key factors explaining why woody plants are less capable of tolerating both shade and drought in habitats where vegetation period is relatively short and water table high. While dormancy enables angiosperms to more successfully face additional stress factors besides shade and drought, gymnosperms have lower polytolerance, but are better tolerators of shade and drought when other environmental factors are favorable.

Dataset Information

Abiotic stresses influence the transcript abundance of PIP and TIP aquaporins in Festuca species.

Publications

Abiotic stresses influence the transcript abundance of PIP and TIP aquaporins in Festuca species.

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