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PIP₃ abundance overcomes PI3K signaling selectivity in invadopodia.

ABSTRACT: PI3Kβ is required for invadopodia-mediated matrix degradation by breast cancer cells. Invadopodia maturation requires GPCR activation of PI3Kβ and its coupling to SHIP2 to produce PI(3,4)P₂ . We now test whether selectivity for PI3Kβ is preserved under conditions of mutational increases in PI3K activity. In breast cancer cells where PI3Kβ is inhibited, short-chain diC8-PIP₃ rescues gelatin degradation in a SHIP2-dependent manner; rescue by diC8-PI(3,4)P₂ is SHIP2-independent. Surprisingly, the expression of either activated PI3Kβ or PI3Kα mutants rescued the effects of PI3Kβ inhibition. In both cases, gelatin degradation was SHIP2-dependent. These data confirm the requirement for PIP₃ conversion to PI(3,4)P₂ for invadopodia function and suggest that selectivity for distinct PI3K isotypes may be obviated by mutational activation of the PI3K pathway.

SUBMITTER: Jakubik CT

PROVIDER: S-EPMC8885911 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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PIP3 abundance overcomes PI3K signaling selectivity in invadopodia.

Jakubik Charles T CT Weckerly Claire C CC Hammond Gerald R V GRV Bresnick Anne R AR Backer Jonathan M JM

FEBS letters 20220112 4

PI3Kβ is required for invadopodia-mediated matrix degradation by breast cancer cells. Invadopodia maturation requires GPCR activation of PI3Kβ and its coupling to SHIP2 to produce PI(3,4)P2 . We now test whether selectivity for PI3Kβ is preserved under conditions of mutational increases in PI3K activity. In breast cancer cells where PI3Kβ is inhibited, short-chain diC8-PIP3 rescues gelatin degradation in a SHIP2-dependent manner; rescue by diC8-PI(3,4)P2 is SHI ...[more]

PMID: 34990021

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