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MiR-204 is associated with an endocrine phenotype in human pancreatic islets but does not regulate the insulin mRNA through MAFA.


ABSTRACT: miR-204 has been proposed to modulate insulin expression in human pancreatic islets by regulating the expression of the MAFA transcript, and in turn insulin transcription. We investigated miR-204 expression in pancreatic endocrine tumors (PET), a panel of human tissues, tissues derived from pancreatic islet purification, and in induced pluripotent stem cells (iPSCs) differentiated towards a pancreatic endocrine phenotype by quantitative real time RT-PCR or droplet digital PCR (ddPCR). In addition, we evaluated the effect of miR-204 up- or down-regulation in purified human islets and in the EndoC-?H1 cell line, as an experimental model of human pancreatic ? cells. Our results confirm that miR-204 was enriched in insulin producing PET, in ? cells within healthy pancreatic islets, and highly expressed in EndoC-?H1 cells. Moreover, in iPSCs miR-204 increased stepwise upon stimulated differentiation to insulin producing cells. However, up- or down-regulation of miR-204 in human islets and in EndoC-?H1 cells resulted in modest and not significant changes of the MAFA and INS mRNAs measured by ddPCR or c-peptide release. Our data confirm the association of miR-204 with a ? cell endocrine phenotype in human pancreatic islets, but do not support its direct role in regulating the levels of insulin mRNA through MAFA.

SUBMITTER: Marzinotto I 

PROVIDER: S-EPMC5656581 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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miR-204 is associated with an endocrine phenotype in human pancreatic islets but does not regulate the insulin mRNA through MAFA.

Marzinotto Ilaria I   Pellegrini Silvia S   Brigatti Cristina C   Nano Rita R   Melzi Raffaella R   Mercalli Alessia A   Liberati Daniela D   Sordi Valeria V   Ferrari Maurizio M   Falconi Massimo M   Doglioni Claudio C   Ravassard Philippe P   Piemonti Lorenzo L   Lampasona Vito V  

Scientific reports 20171025 1


miR-204 has been proposed to modulate insulin expression in human pancreatic islets by regulating the expression of the MAFA transcript, and in turn insulin transcription. We investigated miR-204 expression in pancreatic endocrine tumors (PET), a panel of human tissues, tissues derived from pancreatic islet purification, and in induced pluripotent stem cells (iPSCs) differentiated towards a pancreatic endocrine phenotype by quantitative real time RT-PCR or droplet digital PCR (ddPCR). In additio  ...[more]

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