New reverse genetics and transfection methods to rescue arboviruses in mosquito cells.
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ABSTRACT:
SUBMITTER: Atieh T
PROVIDER: S-EPMC5656662 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Publications
New reverse genetics and transfection methods to rescue arboviruses in mosquito cells.
Atieh Thérèse T Nougairède Antoine A Klitting Raphaëlle R Aubry Fabien F Failloux Anna-Bella AB de Lamballerie Xavier X Priet Stéphane S
Scientific reports 20171025 1
Reverse genetics is a critical tool to decrypt the biological properties of arboviruses. However, whilst reverse genetics methods have been usually applied to vertebrate cells, their use in insect cells remains uncommon due to the conjunction of laborious molecular biology techniques and of specific difficulties surrounding the transfection of such cells. To leverage reverse genetics studies in both vertebrate and mosquito cells, we designed an improved DNA transfection protocol for insect cells ...[more]
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