Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats.
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ABSTRACT:
SUBMITTER: Mazza M
PROVIDER: S-EPMC5656779 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Publications
Low fraction of the 222K PrP variant in the protease-resistant moiety of PrPres in heterozygous scrapie positive goats.
Mazza Maria M Guglielmetti Chiara C Ingravalle Francesco F Brusadore Sonia S Langeveld Jan P M JPM Ekateriniadou Loukia V LV Andréoletti Olivier O Casalone Cristina C Acutis Pier Luigi PL
The Journal of general virology 20170713 7
The presence of lysine (K) at codon 222 has been associated with resistance to classical scrapie in goats, but few scrapie cases have been identified in 222Q/K animals. To investigate the contribution of the 222K variant to PrPres formation in natural and experimental Q/K scrapie cases, we applied an immunoblotting method based on the use of two different monoclonal antibodies, F99/97.6.1 and SAF84, chosen for their different affinities to 222K and 222Q PrP variants. Our finding that PrPres seem ...[more]
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