Ontology highlight
ABSTRACT:
SUBMITTER: Chen X
PROVIDER: S-EPMC5658784 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Chen Xiaofei X Wu Hanzhi H Park Chung-Min CM Poole Thomas H TH Keceli Gizem G Devarie-Baez Nelmi O NO Tsang Allen W AW Lowther W Todd WT Poole Leslie B LB King S Bruce SB Xian Ming M Furdui Cristina M CM
ACS chemical biology 20170719 8
The selective reaction of chemical reagents with reduced protein thiols is critical to biological research. This reaction is utilized to prevent cross-linking of cysteine-containing peptides in common proteomics workflows and is applied widely in discovery and targeted redox investigations of the mechanisms underlying physiological and pathological processes. However, known and commonly used thiol blocking reagents like iodoacetamide, N-ethylmaleimide, and others were found to cross-react with o ...[more]