Ontology highlight
ABSTRACT:
SUBMITTER: Cowell J
PROVIDER: S-EPMC5708275 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Chembiochem : a European journal of chemical biology 20170719 17
Biotinylation of amines is widely used to conjugate biomolecules, but either the resulting label is non-removable or its removal leaves a tag on the molecule of interest, thus affecting downstream processes. We present here a set of reagents (RevAmines) that allow traceless, reversible biotinylation under biologically compatible, mild conditions. Release following avidin-based capture is achieved through the cleavage of a (2-(alkylsulfonyl)ethyl) carbamate linker under mild conditions (200 mm am ...[more]