Polydatin reduces Staphylococcus aureus lipoteichoic acid-induced injury by attenuating reactive oxygen species generation and TLR2-NF?B signalling.

ABSTRACT: Staphylococcus aureus (S. aureus) causes severe inflammation in various infectious diseases, leading to high mortality. The clinical application of antibiotics has gained a significant curative effect. However, it has led to the emergence of various resistant bacteria. Therefore, in this study, we investigated the protective effect of polydatin (PD), a traditional Chinese medicine extract, on S. aureus lipoteichoic acid (LTA)-induced injury in vitro and in vivo. First, a significant improvement in the pathological conditions of PD in vivo was observed, suggesting that PD had a certain protective effect on LTA-induced injury in a mouse model. To further explore the underlying mechanisms of this protective effect of PD, LTA-induced murine macrophages were used in this study. The results have shown that PD could reduce the NF-?B p65, and I?B? phosphorylation levels increased by LTA, resulting in a decrease in the transcription of pro-inflammatory factors, such as TNF-?, IL-1? and IL-6. However, LTA can not only activate NF-?B through the recognition of TLR2 but also increase the level of intracellular reactive oxygen species (ROS), thereby activating NF-?B signalling. We also detected high levels of ROS that activate caspases 9 and 3 to induce apoptosis. In addition, using a specific NF-?B inhibitor that could attenuate apoptosis, namely NF-?B p65, acted as a pro-apoptotic transcription factor in LTA-induced murine macrophages. However, PD could inhibit the generation of ROS and NF-?B p65 activation, suggesting that PD suppressed LTA-induced injury by attenuating ROS generation and TLR2-NF?B signalling.

SUBMITTER: Zhao G 

PROVIDER: S-EPMC5661256 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature