Ontology highlight
ABSTRACT:
SUBMITTER: Kumagai A
PROVIDER: S-EPMC5662258 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Kumagai Akiko A Dunphy William G WG
Molecular biology of the cell 20170906 22
Treslin, which is essential for incorporation of Cdc45 into the replicative helicase, possesses a partner called MTBP (Mdm2-binding protein). We have analyzed <i>Xenopus</i> and human MTBP to assess its role in DNA replication. Depletion of MTBP from <i>Xenopus</i> egg extracts, which also removes Treslin, abolishes DNA replication. These extracts be can rescued with recombinant Treslin-MTBP but not Treslin or MTBP alone. Thus, Treslin-MTBP is collectively necessary for replication. We have iden ...[more]