Dataset Information

Membrane dynamics and organelle biogenesis-lipid pipelines and vesicular carriers.

ABSTRACT: Discoveries spanning several decades have pointed to vital membrane lipid trafficking pathways involving both vesicular and non-vesicular carriers. But the relative contributions for distinct membrane delivery pathways in cell growth and organelle biogenesis continue to be a puzzle. This is because lipids flow from many sources and across many paths via transport vesicles, non-vesicular transfer proteins, and dynamic interactions between organelles at membrane contact sites. This forum presents our latest understanding, appreciation, and queries regarding the lipid transport mechanisms necessary to drive membrane expansion during organelle biogenesis and cell growth.

SUBMITTER: Stefan CJ

PROVIDER: S-EPMC5663033 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Publications

Membrane dynamics and organelle biogenesis-lipid pipelines and vesicular carriers.

Stefan Christopher J CJ Trimble William S WS Grinstein Sergio S Drin Guillaume G Reinisch Karin K De Camilli Pietro P Cohen Sarah S Valm Alex M AM Lippincott-Schwartz Jennifer J Levine Tim P TP Iaea David B DB Maxfield Frederick R FR Futter Clare E CE Eden Emily R ER Judith Delphine D van Vliet Alexander R AR Agostinis Patrizia P Tooze Sharon A SA Sugiura Ayumu A McBride Heidi M HM

BMC biology 20171031 1

Discoveries spanning several decades have pointed to vital membrane lipid trafficking pathways involving both vesicular and non-vesicular carriers. But the relative contributions for distinct membrane delivery pathways in cell growth and organelle biogenesis continue to be a puzzle. This is because lipids flow from many sources and across many paths via transport vesicles, non-vesicular transfer proteins, and dynamic interactions between organelles at membrane contact sites. This forum presents ...[more]

PMID: 29089042

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Project description:Trichomonas vaginalis is a parasitic protist of the Excavata group. It contains an anaerobic form of mitochondria called hydrogenosomes, which produce hydrogen and ATP; the majority of mitochondrial pathways and the organellar genome were lost during the mitochondrion-to-hydrogenosome transition. Consequently, all hydrogenosomal proteins are encoded in the nucleus and imported into the organelles. However, little is known about the membrane machineries required for biogenesis of the organelle and metabolite exchange. Using a combination of mass spectrometry, immunofluorescence microscopy, in vitro import assays and reverse genetics, we characterized the membrane proteins of the hydrogenosome. We identified components of the outer membrane (TOM) and inner membrane (TIM) protein translocases include multiple paralogs of the core Tom40-type porins and Tim17/22/23 channel proteins, respectively, and uniquely modified small Tim chaperones. The inner membrane proteins TvTim17/22/23-1 and Pam18 were shown to possess conserved information for targeting to mitochondrial inner membranes, but too divergent in sequence to support the growth of yeast strains lacking Tim17, Tim22, Tim23 or Pam18. Full complementation was seen only when the J-domain of hydrogenosomal Pam18 was fused with N-terminal region and transmembrane segment of the yeast homolog. Candidates for metabolite exchange across the outer membrane were identified including multiple isoforms of the ?-barrel proteins, Hmp35 and Hmp36; inner membrane MCF-type metabolite carriers were limited to five homologs of the ATP/ADP carrier, Hmp31. Lastly, hydrogenosomes possess a pathway for the assembly of C-tail-anchored proteins into their outer membrane with several new tail-anchored proteins being identified. These results show that hydrogenosomes and mitochondria share common core membrane components required for protein import and metabolite exchange; however, they also reveal remarkable differences that reflect the functional adaptation of hydrogenosomes to anaerobic conditions and the peculiar evolutionary history of the Excavata group.

Dataset Information

Membrane dynamics and organelle biogenesis-lipid pipelines and vesicular carriers.

Publications

Membrane dynamics and organelle biogenesis-lipid pipelines and vesicular carriers.

Similar Datasets

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