Quantitative 3D-imaging for cell biology and ecology of environmental microbial eukaryotes.
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ABSTRACT:
SUBMITTER: Colin S
PROVIDER: S-EPMC5663481 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Publications
Quantitative 3D-imaging for cell biology and ecology of environmental microbial eukaryotes.
Colin Sebastien S Coelho Luis Pedro LP Sunagawa Shinichi S Bowler Chris C Karsenti Eric E Bork Peer P Pepperkok Rainer R de Vargas Colomban C
eLife 20171031
We present a 3D-fluorescence imaging and classification tool for high throughput analysis of microbial eukaryotes in environmental samples. It entails high-content feature extraction that permits accurate automated taxonomic classification and quantitative data about organism ultrastructures and interactions. Using plankton samples from the <i>Tara</i> Oceans expeditions, we validate its applicability to taxonomic profiling and ecosystem analyses, and discuss its potential for future integration ...[more]
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