Unknown

Dataset Information

0

MicroRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma.


ABSTRACT: Glycolysis was reported to have a positive correlation with radioresistance. Our previous study found that the miR-33a functioned as a tumor suppressor in malignant melanoma by targeting hypoxia-inducible factor1-alpha (HIF-1?), a gene known to promote glycolysis. However, the role of miR-33a-5p in radiosensitivity remains to be elucidated. We found that miR-33a-5p was downregulated in melanoma tissues and cells. Cell proliferation was downregulated after overexpression of miR-33a-5p in WM451 cells, accompanied by a decreased level of glycolysis. In contrast, cell proliferation was upregulated after inhibition of miR-33a-5p in WM35 cells, accompanied by increased glycolysis. Overexpression of miR-33a-5p enhanced the sensitivity of melanoma cells to X-radiation by MTT assay, while downregulation of miR-33a-5p had the opposite effects. Finally, in vivo experiments with xenografts in nude mice confirmed that high expression of miR-33a-5p in tumor cells increased radiosensitivity via inhibiting glycolysis. In conclusions, miR-33a-5p promotes radiosensitivity by negatively regulating glycolysis in melanoma.

SUBMITTER: Cao K 

PROVIDER: S-EPMC5663544 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

microRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma.

Cao Ke K   Li Jingjing J   Chen Jia J   Qian Li L   Wang Aijun A   Chen Xiang X   Xiong Wei W   Tang Jintian J   Tang Shijie S   Chen Yong Y   Chen Yao Y   Cheng Yan Y   Zhou Jianda J  

Oncotarget 20170705 48


Glycolysis was reported to have a positive correlation with radioresistance. Our previous study found that the miR-33a functioned as a tumor suppressor in malignant melanoma by targeting hypoxia-inducible factor1-alpha (<i>HIF-1α</i>), a gene known to promote glycolysis. However, the role of miR-33a-5p in radiosensitivity remains to be elucidated. We found that miR-33a-5p was downregulated in melanoma tissues and cells. Cell proliferation was downregulated after overexpression of miR-33a-5p in W  ...[more]

Similar Datasets

| S-EPMC4794666 | biostudies-literature
| S-EPMC7171649 | biostudies-literature
| S-EPMC9852070 | biostudies-literature
| S-EPMC5630345 | biostudies-literature
| S-EPMC5793389 | biostudies-literature
| S-EPMC7352388 | biostudies-literature
| S-EPMC7399786 | biostudies-literature
| S-EPMC7933330 | biostudies-literature
| S-EPMC9077299 | biostudies-literature
| S-EPMC6770036 | biostudies-literature