Ontology highlight
ABSTRACT:
SUBMITTER: Li J
PROVIDER: S-EPMC5663727 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Li Jiyun J Shi Xiaomin X Yin Wenjuan W Wang Yang Y Shen Zhangqi Z Ding Shuangyang S Wang Shaolin S
Frontiers in microbiology 20171027
The aim of the study was to develop a multiplex assay for rapid detection of <i>mcr-1, mcr-2</i>, and <i>mcr-3</i>, a group of genes of conferring resistance to colistin mediated by plasmid in Enterobacteriaceae. A SYBR Green based real-time PCR assay has been designed to detect the <i>mcr</i> genes, and applied to cultured bacteria, feces and soil samples. All three <i>mcr</i> genes could be detected with a lower limit of 10<sup>2</sup> cultured bacteria. This test was highly specific and sensi ...[more]