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Simultaneous epitope and transcriptome measurement in single cells.

ABSTRACT: High-throughput single-cell RNA sequencing has transformed our understanding of complex cell populations, but it does not provide phenotypic information such as cell-surface protein levels. Here, we describe cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), a method in which oligonucleotide-labeled antibodies are used to integrate cellular protein and transcriptome measurements into an efficient, single-cell readout. CITE-seq is compatible with existing single-cell sequencing approaches and scales readily with throughput increases.

SUBMITTER: Stoeckius M

PROVIDER: S-EPMC5669064 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Simultaneous epitope and transcriptome measurement in single cells.

Stoeckius Marlon M Hafemeister Christoph C Stephenson William W Houck-Loomis Brian B Chattopadhyay Pratip K PK Swerdlow Harold H Satija Rahul R Smibert Peter P

Nature methods 20170731 9

High-throughput single-cell RNA sequencing has transformed our understanding of complex cell populations, but it does not provide phenotypic information such as cell-surface protein levels. Here, we describe cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), a method in which oligonucleotide-labeled antibodies are used to integrate cellular protein and transcriptome measurements into an efficient, single-cell readout. CITE-seq is compatible with existing single-cell seque ...[more]

PMID: 28759029

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