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Simultaneous measurement of biochemical phenotypes and gene expression in single cells.

ABSTRACT: Methods to measure heterogeneity among cells are rapidly transforming our understanding of biology but are currently limited to molecular abundance measurements. We developed an approach to simultaneously measure biochemical activities and mRNA abundance in single cells to understand the heterogeneity of DNA repair activities across thousands of human lymphocytes, identifying known and novel cell-type-specific DNA repair phenotypes.

SUBMITTER: Richer AL

PROVIDER: S-EPMC7261187 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Simultaneous measurement of biochemical phenotypes and gene expression in single cells.

Richer Amanda L AL Riemondy Kent A KA Hardie Lakotah L Hesselberth Jay R JR

Nucleic acids research 20200601 10

Methods to measure heterogeneity among cells are rapidly transforming our understanding of biology but are currently limited to molecular abundance measurements. We developed an approach to simultaneously measure biochemical activities and mRNA abundance in single cells to understand the heterogeneity of DNA repair activities across thousands of human lymphocytes, identifying known and novel cell-type-specific DNA repair phenotypes. ...[more]

PMID: 32286626

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