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Complete genome sequences of two insect-specific flaviviruses.

ABSTRACT: We determined the complete genomic sequences of two previously discovered insect-specific flaviviruses, Marisma mosquito virus (MMV) and Nanay virus (NANV), using a combination of high-throughput sequencing, reverse transcription-polymerase chain reaction, 5' and 3' rapid amplification of cDNA ends and Sanger sequencing. Complete polyprotein amino acid sequence alignments revealed that the closest known relatives of MMV and NANV are Donggang virus (89% identity, 95% similarity) and Nounané virus (53% identity, 70% similarity), respectively. Phylogenetic inference is in agreement with these findings. Potential programmed -1 ribosomal frameshifting sites were bioinformatically identified in the genomes of both viruses.

SUBMITTER: Charles J

PROVIDER: S-EPMC5671525 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Complete genome sequences of two insect-specific flaviviruses.

Charles Jermilia J Tangudu Chandra S CS Firth Andrew E AE Blitvich Bradley J BJ

Archives of virology 20170916 12

We determined the complete genomic sequences of two previously discovered insect-specific flaviviruses, Marisma mosquito virus (MMV) and Nanay virus (NANV), using a combination of high-throughput sequencing, reverse transcription-polymerase chain reaction, 5' and 3' rapid amplification of cDNA ends and Sanger sequencing. Complete polyprotein amino acid sequence alignments revealed that the closest known relatives of MMV and NANV are Donggang virus (89% identity, 95% similarity) and Nounané virus ...[more]

PMID: 28918473

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