Ontology highlight
ABSTRACT:
SUBMITTER: Milligan L
PROVIDER: S-EPMC5673307 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Milligan Laura L Sayou Camille C Tuck Alex A Auchynnikava Tatsiana T Reid Jane Ea JE Alexander Ross R Alves Flavia de Lima FL Allshire Robin R Spanos Christos C Rappsilber Juri J Beggs Jean D JD Kudla Grzegorz G Tollervey David D
eLife 20171013
Numerous links exist between co-transcriptional RNA processing and the transcribing RNAPII. In particular, pre-mRNA splicing was reported to be associated with slowed RNAPII elongation. Here, we identify a site of ubiquitination (K1246) in the catalytic subunit of RNAPII close to the DNA entry path. Ubiquitination was increased in the absence of the Bre5-Ubp3 ubiquitin protease complex. Bre5 binds RNA in vivo, with a preference for exon 2 regions of intron-containing pre-mRNAs and poly(A) proxim ...[more]