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Specific Light-Up System for Protein and Metabolite Targets Triggered by Initiation Complex Formation.

ABSTRACT: Gene regulation systems are mimicked by simple quantitative detection of non-nucleic acid molecular targets such as protein and metabolite. Here, we describe a one-tube, one-step real-time quantitative detection methodology for isothermal signal amplification of those targets. Using this system, real-time quantitative detection of thrombin and streptomycin, which were used as examples for protein and metabolite targets, was successfully demonstrated with detection limits of at most 50 pM and 75?nM, respectively. Notably, the dynamic range of target concentrations could be obtained for over four orders of magnitude. Thus, our method is expected to serve as a point-of-care or on-site test for medical diagnosis and food and environmental hygiene.

SUBMITTER: Fujita H

PROVIDER: S-EPMC5680199 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Specific Light-Up System for Protein and Metabolite Targets Triggered by Initiation Complex Formation.

Fujita Hiroto H Kataoka Yuka Y Nagano Remi R Nakajima Yasuyo Y Yamada Masanobu M Sugimoto Naoki N Kuwahara Masayasu M

Scientific reports 20171109 1

Gene regulation systems are mimicked by simple quantitative detection of non-nucleic acid molecular targets such as protein and metabolite. Here, we describe a one-tube, one-step real-time quantitative detection methodology for isothermal signal amplification of those targets. Using this system, real-time quantitative detection of thrombin and streptomycin, which were used as examples for protein and metabolite targets, was successfully demonstrated with detection limits of at most 50 pM and 75 ...[more]

PMID: 29123195

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