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A Novel Cyclophilin B Gene in the Red Tide Dinoflagellate Cochlodinium polykrikoides: Molecular Characterizations and Transcriptional Responses to Environmental Stresses.


ABSTRACT: The marine dinoflagellate Cochlodinium polykrikoides is one of the most common ichthyotoxic species that causes harmful algal blooms (HABs), which leads to ecological damage and huge economic loss in aquaculture industries. Cyclophilins (CYPs) belong to the immunophilin superfamily, and they may play a role in the survival mechanisms of the dinoflagellate in stress environments. In the present study, we identified a novel cyclophilin gene from C. polykrikoides and examined physiological and gene transcriptional responses to biocides copper sulphate (CuSO4) and sodium hypochlorite (NaOCl). The full length of CpCYP was 903?bp, ranging from the dinoflagellate splice leader (DinoSL) sequence to the polyA tail, comprising a 639?bp ORF, a 117?bp 5'-UTR, and a 147?bp 3'-UTR. Motif and phylogenetic comparisons showed that CpCYP was affiliated to group B of CYP. In biocide stressors, cell counts, chlorophyll a, and photosynthetic efficiency (Fv/Fm) of C. polykrikoides were considerably decreased in both exposure time- and dose-dependent manners. In addition, CpCYP gene expression was significantly induced after 24?h exposure to the biocide-treated stress conditions. These results indicate an effect of the biocides on the cell physiology and expression profile of CpCYP, suggesting that the gene may play a role in environmental stress responses.

SUBMITTER: Abassi S 

PROVIDER: S-EPMC5684524 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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A Novel Cyclophilin B Gene in the Red Tide Dinoflagellate <i>Cochlodinium polykrikoides</i>: Molecular Characterizations and Transcriptional Responses to Environmental Stresses.

Abassi Sofia S   Wang Hui H   Park Bum Soo BS   Park Jong-Woo JW   Ki Jang-Seu JS  

BioMed research international 20171026


The marine dinoflagellate <i>Cochlodinium polykrikoides</i> is one of the most common ichthyotoxic species that causes harmful algal blooms (HABs), which leads to ecological damage and huge economic loss in aquaculture industries. Cyclophilins (CYPs) belong to the immunophilin superfamily, and they may play a role in the survival mechanisms of the dinoflagellate in stress environments. In the present study, we identified a novel cyclophilin gene from <i>C. polykrikoides</i> and examined physiolo  ...[more]

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