Ontology highlight
ABSTRACT:
SUBMITTER: Sabirov RZ
PROVIDER: S-EPMC5686547 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Sabirov Ravshan Z RZ Merzlyak Petr G PG Okada Toshiaki T Islam Md Rafiqul MR Uramoto Hiromi H Mori Tomoko T Makino Yumiko Y Matsuura Hiroshi H Xie Yu Y Okada Yasunobu Y
The EMBO journal 20171018 22
The maxi-anion channels (MACs) are expressed in cells from mammals to amphibians with ~60% exhibiting a phenotype called Maxi-Cl. Maxi-Cl serves as the most efficient pathway for regulated fluxes of inorganic and organic anions including ATP However, its molecular entity has long been elusive. By subjecting proteins isolated from bleb membranes rich in Maxi-Cl activity to LC-MS/MS combined with targeted siRNA screening, CRISPR/Cas9-mediated knockout, and heterologous overexpression, we identifie ...[more]