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LRPPRC-mediated folding of the mitochondrial transcriptome.


ABSTRACT: The expression of the compact mammalian mitochondrial genome requires transcription, RNA processing, translation and RNA decay, much like the more complex chromosomal systems, and here we use it as a model system to understand the fundamental aspects of gene expression. Here we combine RNase footprinting with PAR-CLIP at unprecedented depth to reveal the importance of RNA-protein interactions in dictating RNA folding within the mitochondrial transcriptome. We show that LRPPRC, in complex with its protein partner SLIRP, binds throughout the mitochondrial transcriptome, with a preference for mRNAs, and its loss affects the entire secondary structure and stability of the transcriptome. We demonstrate that the LRPPRC-SLIRP complex is a global RNA chaperone that stabilizes RNA structures to expose the required sites for translation, stabilization, and polyadenylation. Our findings reveal a general mechanism where extensive RNA-protein interactions ensure that RNA is accessible for its biological functions.

SUBMITTER: Siira SJ 

PROVIDER: S-EPMC5691074 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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LRPPRC-mediated folding of the mitochondrial transcriptome.

Siira Stefan J SJ   Spåhr Henrik H   Shearwood Anne-Marie J AJ   Ruzzenente Benedetta B   Larsson Nils-Göran NG   Rackham Oliver O   Filipovska Aleksandra A  

Nature communications 20171116 1


The expression of the compact mammalian mitochondrial genome requires transcription, RNA processing, translation and RNA decay, much like the more complex chromosomal systems, and here we use it as a model system to understand the fundamental aspects of gene expression. Here we combine RNase footprinting with PAR-CLIP at unprecedented depth to reveal the importance of RNA-protein interactions in dictating RNA folding within the mitochondrial transcriptome. We show that LRPPRC, in complex with it  ...[more]

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