Ontology highlight
ABSTRACT:
SUBMITTER: Hao N
PROVIDER: S-EPMC5696343 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Nature communications 20171120 1
DNA looping is a ubiquitous and critical feature of gene regulation. Although DNA looping can be efficiently detected, tools to readily manipulate DNA looping are limited. Here we develop CRISPR-based DNA looping reagents for creation of programmable DNA loops. Cleavage-defective Cas9 proteins of different specificity are linked by heterodimerization or translational fusion to create bivalent complexes able to link two separate DNA regions. After model-directed optimization, the reagents are val ...[more]