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Characterization of wheat MYB genes responsive to high temperatures.

ABSTRACT: Heat stress is one of the most crucial environmental factors, which reduces crop yield worldwide. In plants, the MYB family is one of the largest families of transcription factors (TFs). Although some wheat stress-related MYB TFs have been characterized, their involvement in response to high-temperature stress has not been properly studied.Six novel heat-induced MYB genes were identified by comparison with previously established de novo transcriptome sequencing data obtained from wheat plants subjected to heat treatment; genomic and complete coding sequences of these genes were isolated. All six TaMYBs were localized in the nucleus of wheat protoplasts. Transactivation assays in yeast revealed that all six proteins acted as transcriptional activators, and the activation domains were attributed to the C-termini of the six wheat MYB proteins. Phylogenetic analysis of the six TaMYBs and R2R3-MYBs from Arabidopsis revealed that all six proteins were in clades that contained stress-related MYB TFs. The expression profiles of TaMYB genes were different in wheat tissues and in response to various abiotic stresses and exogenous abscisic acid treatment. In transgenic Arabidopsis plants carrying TaMYB80 driven by the CaMV 35S promoter, tolerance to heat and drought stresses increased, which could be attributed to the increased levels of cellular abscisic acid.We identified six heat-induced MYB genes in wheat. We performed comprehensive analyses of the cloned MYB genes and their gene products, including gene structures, subcellular localization, transcriptional activation, phylogenetic relationships, and expression patterns in different wheat tissues and under various abiotic stresses. In particular, we showed that TaMYB80 conferred heat and drought tolerance in transgenic Arabidopsis. These results contribute to our understanding of the functions of heat-induced MYB genes and provide the basis for selecting the best candidates for in-depth functional studies of heat-responsive MYB genes in wheat.

SUBMITTER: Zhao Y

PROVIDER: S-EPMC5696766 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Characterization of wheat MYB genes responsive to high temperatures.

Zhao Yue Y Tian Xuejun X Wang Fei F Zhang Liyuan L Xin Mingming M Hu Zhaorong Z Yao Yingyin Y Ni Zhongfu Z Sun Qixin Q Peng Huiru H

BMC plant biology 20171121 1

<h4>Background</h4>Heat stress is one of the most crucial environmental factors, which reduces crop yield worldwide. In plants, the MYB family is one of the largest families of transcription factors (TFs). Although some wheat stress-related MYB TFs have been characterized, their involvement in response to high-temperature stress has not been properly studied.<h4>Results</h4>Six novel heat-induced MYB genes were identified by comparison with previously established de novo transcriptome sequencing ...[more]

PMID: 29157199

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Project description:Effective chromosome synapsis and crossover formation during meiosis are essential for fertility, especially in grain crops such as wheat. These processes function most efficiently in wheat at temperatures between 17-23 °C, although the genetic mechanisms for such temperature dependence are unknown. In a previously identified mutant of the hexaploid wheat reference variety 'Chinese Spring' lacking the long arm of chromosome 5D, exposure to low temperatures during meiosis resulted in asynapsis and crossover failure. In a second mutant (ttmei1), containing a 4 Mb deletion in chromosome 5DL, exposure to 13 °C led to similarly high levels of asynapsis and univalence. Moreover, exposure to 30 °C led to a significant, but less extreme effect on crossovers. Previously, we proposed that, of 41 genes deleted in this 4 Mb region, the major meiotic gene TaDMC1-D1 was the most likely candidate for preservation of synapsis and crossovers at low (and possibly high) temperatures. In the current study, using RNA-guided Cas9, we developed a new Chinese Spring CRISPR mutant, containing a 39 bp deletion in the 5D copy of DMC1, representing the first reported CRISPR-Cas9 targeted mutagenesis in Chinese Spring, and the first CRISPR mutant for DMC1 in wheat. In controlled environment experiments, wild-type Chinese Spring, CRISPR dmc1-D1 and backcrossed ttmei1 mutants were exposed to either high or low temperatures during the temperature-sensitive period from premeiotic interphase to early meiosis I. After 6-7 days at 13 °C, crossovers decreased by over 95% in the dmc1-D1 mutants, when compared with wild-type plants grown under the same conditions. After 24 hours at 30 °C, dmc1-D1 mutants exhibited a reduced number of crossovers and increased univalence, although these differences were less marked than at 13 °C. Similar results were obtained for ttmei1 mutants, although their scores were more variable, possibly reflecting higher levels of background mutation. These experiments confirm our previous hypothesis that DMC1-D1 is responsible for preservation of normal crossover formation at low and, to a certain extent, high temperatures. Given that reductions in crossovers have significant effects on grain yield, these results have important implications for wheat breeding, particularly in the face of climate change.

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Characterization of wheat MYB genes responsive to high temperatures.

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Characterization of wheat MYB genes responsive to high temperatures.

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