Ontology highlight
ABSTRACT:
SUBMITTER: Olsen OE
PROVIDER: S-EPMC5697882 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Olsen Oddrun Elise OE Skjærvik Anette A Størdal Berit Fladvad BF Sundan Anders A Holien Toril T
PloS one 20171121 11
Purified recombinant proteins for use in biomedical research are invaluable to investigate protein function. However, purity varies in protein batches made in mammalian expression systems, such as CHO-cells or HEK293-cells. This study points to caution while investigating effects of proteins related to the transforming growth factor (TGF)-β superfamily. TGF-β itself is a very potent cytokine and has effects on cells in the femtomolar range. Thus, even very small amounts of contaminating TGF-β in ...[more]