A CRISPR screen identifies a pathway required for paraquat-induced cell death.
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ABSTRACT:
SUBMITTER: Reczek CR
PROVIDER: S-EPMC5698099 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
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A CRISPR screen identifies a pathway required for paraquat-induced cell death.
Reczek Colleen R CR Birsoy Kıvanç K Kong Hyewon H Martínez-Reyes Inmaculada I Wang Tim T Gao Peng P Sabatini David M DM Chandel Navdeep S NS
Nature chemical biology 20171023 12
Paraquat, a herbicide linked to Parkinson's disease, generates reactive oxygen species (ROS), which causes cell death. Because the source of paraquat-induced ROS production remains unknown, we conducted a CRISPR-based positive-selection screen to identify metabolic genes essential for paraquat-induced cell death. Our screen uncovered three genes, POR (cytochrome P450 oxidoreductase), ATP7A (copper transporter), and SLC45A4 (sucrose transporter), required for paraquat-induced cell death. Furtherm ...[more]
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