Ontology highlight
ABSTRACT:
SUBMITTER: Carlson-Stevermer J
PROVIDER: S-EPMC5700129 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Carlson-Stevermer Jared J Abdeen Amr A AA Kohlenberg Lucille L Goedland Madelyn M Molugu Kaivalya K Lou Meng M Saha Krishanu K
Nature communications 20171123 1
Writing specific DNA sequences into the human genome is challenging with non-viral gene-editing reagents, since most of the edited sequences contain various imprecise insertions or deletions. We developed a modular RNA aptamer-streptavidin strategy, termed S1mplex, to complex CRISPR-Cas9 ribonucleoproteins with a nucleic acid donor template, as well as other biotinylated molecules such as quantum dots. In human cells, tailored S1mplexes increase the ratio of precisely edited to imprecisely edite ...[more]